Microbiology

6 subjects · 13 chapters · 52 topics

1. 01

   The Microbial World

   1.1 Introduction

   • History and scope of microbiology
   • Classification of microorganisms
   • Prokaryotes versus eukaryotes
   • Microbial diversity

   1.2 Microscopy

   • Light, phase contrast and fluorescence
   • Electron microscopy
   • Staining: simple, differential, special
   • Measuring microbes
2. 02

   Structure and Growth

   2.1 Cell structure

   • Bacterial cell wall and Gram reaction
   • Capsules, flagella and pili
   • Spores and survival structures
   • Fungal and protozoan structure

   2.2 Growth and culture

   • Growth curve and generation time
   • Nutritional requirements
   • Media types and preparation
   • Aseptic technique and pure culture

   2.3 Control of microbes

   • Sterilisation methods
   • Disinfectants and antiseptics
   • Antibiotics and their mechanisms
   • Antimicrobial resistance
3. 03

   Microbial Genetics and Metabolism

   3.1 Metabolism

   • Respiration and fermentation
   • Autotrophy and heterotrophy
   • Enzymes and pathways
   • Energy yield

   3.2 Genetics

   • Bacterial genome and plasmids
   • Mutation and repair
   • Transformation, transduction, conjugation
   • Genetic tools in microbiology
4. 04

   Applied Microbiology

   4.1 Medical microbiology

   • Host-pathogen interaction
   • Major bacterial pathogens
   • Viruses and viral disease
   • Immunity and vaccines

   4.2 Industrial and food

   • Fermentation industries
   • Probiotics and starter cultures
   • Food spoilage and preservation
   • Microbial products and enzymes

   4.3 Environmental

   • Soil and water microbiology
   • Nitrogen and carbon cycles
   • Bioremediation
   • Water quality testing
5. 05

   Laboratory Skills

   5.1 Core techniques

   • Streak, spread and pour plates
   • Biochemical identification tests
   • Antibiotic sensitivity testing
   • Colony counting and dilution

   5.2 Safety and quality

   • Biosafety levels
   • Waste handling
   • Quality control of media
   • Laboratory documentation
6. 06

   Project

   6.1 Investigation

   • Designing a microbiological study
   • Isolating and identifying an organism
   • Interpreting the results
   • Writing the report